8DS8

Crystal structure of human TNRC18 BAH domain in complex with H3K9me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	73.804, 120.828, 46.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.480 - 1.840
R-factor	0.2043
Rwork	0.203
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dov
RMSD bond length	0.009
RMSD bond angle	0.974
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.840	3.990	1.840
Rmerge	0.124	0.053	1.210
Rmeas	0.139	0.059	1.380
Rpim	0.061	0.025	0.649
Number of reflections	36692	3909	3584
<I/σ(I)>	5.2
Completeness [%]	99.5	99.2	99.7
Redundancy	5.2	5.5	4.5
CC(1/2)		0.997	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M Sodium Cacodylate, pH 6.5, 0.2 M Magnesium Acetate, 16-20% PEG 8000