8DPU

The crystal structure of the IL-11 signalling complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 31 1 2
Unit cell lengths	163.411, 163.411, 506.623
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.860 - 3.780
R-factor	0.2765
Rwork	0.276
R-free	0.29510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8dps
RMSD bond length	0.006
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.275	4.197
High resolution limit [Å]	3.779	3.779
Rmeas	0.294	2.584
Rpim	0.064	0.558
Number of reflections	48027	2262
<I/σ(I)>	10.6	1.5
Completeness [%]	92.8
Redundancy	20.9
CC(1/2)	0.998	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	293	180 mM magnesium chloride, 15.3% PEG 3350, 100 mM potassium sodium tartrate, 90 mM sodium HEPES pH 7.25 and 1.8% tert-butanol