8DON

Beta-lactamase CTX-M-14 T215A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11
Spacegroup name	P 32 2 1
Unit cell lengths	41.430, 41.430, 231.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.455 - 1.360
R-factor	0.1457
Rwork	0.145
R-free	0.15950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ylt
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.455	1.409
High resolution limit [Å]	1.360	1.360
Rmerge	0.039	0.069
Rmeas	0.056	0.098
Number of reflections	50984	5041
<I/σ(I)>	11.76	7.92
Completeness [%]	99.6
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Calcium chloride ,0.1 M HEPES pH 7, 20% (w/v) PEG 6000