8DOE
Crystal Structure of CTX-M-14 N106A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.840, 37.910, 65.460 |
| Unit cell angles | 90.00, 103.44, 90.00 |
Refinement procedure
| Resolution | 48.480 - 1.500 |
| R-factor | 0.1456 |
| Rwork | 0.145 |
| R-free | 0.16520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.272 |
| Rmeas | 0.077 | 0.384 |
| Number of reflections | 37847 | 3704 |
| <I/σ(I)> | 9.12 | |
| Completeness [%] | 98.5 | 97.22 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Magnesium chloride ,0.1 M Tris pH 8 20% (w/v), PEG 6000 |
