8DN1

Q108K:K40L:T51C:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V at pH 7.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1272
Spacegroup name	C 1 2 1
Unit cell lengths	29.222, 66.833, 63.704
Unit cell angles	90.00, 91.93, 90.00

Refinement procedure

Resolution	29.590 - 1.320
R-factor	0.19
Rwork	0.188
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qzt
RMSD bond length	0.009
RMSD bond angle	1.156
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.590	1.369
High resolution limit [Å]	1.320	1.320
Rmeas	0.087	0.376
Rpim	0.024	0.134
Number of reflections	28092	2805
<I/σ(I)>	30.8
Completeness [%]	98.1
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	25% PEG 4000, ammonium acetate, sodium acetate pH 4.0 - 4.8