8DJ0
Crystal structure of NavAb L123T as a basis for the human Nav1.7 Inherited Erythromelalgia I848T mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-05-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.970, 124.970, 190.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.180 - 2.700 |
R-factor | 0.2088 |
Rwork | 0.207 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mwa |
RMSD bond length | 0.007 |
RMSD bond angle | 0.925 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.148 | 1.018 |
Rpim | 0.042 | 0.429 |
Number of reflections | 20400 | 746 |
<I/σ(I)> | 14 | 0.4 |
Completeness [%] | 96.5 | 71.4 |
Redundancy | 12 | 4.1 |
CC(1/2) | 1.000 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 1.9 M Ammonium sulfate 0.1 M Sodium Citrate pH 4.6 |