8DIQ

Tubulin-RB3_SLD-TTL in complex with SB226

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 21 21 21
Unit cell lengths	105.491, 158.414, 181.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.395
R-factor	0.1822
Rwork	0.182
R-free	0.20950
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6br1
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.440
High resolution limit [Å]	2.390	2.390
Rmerge	0.088	0.919
Rpim	0.055	0.594
Number of reflections	119484	5742
<I/σ(I)>	12.7	1.8
Completeness [%]	99.7	97.6
Redundancy	6.8	6.4
CC(1/2)	0.998	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4K, MES, Glycerol, CaCl2, MgCl2