8DHY
N-terminal fragment of MsbA fused to GFP in complex with copper(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-07 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.3782 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 70.610, 108.540, 140.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.080 - 2.150 |
R-factor | 0.2029 |
Rwork | 0.202 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.012 |
RMSD bond angle | 1.168 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | XSCALE (Jan 10, 2022) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmeas | 0.094 | 1.450 |
Number of reflections | 56321 | 4109 |
<I/σ(I)> | 12.35 | 1.61 |
Completeness [%] | 99.3 | 98 |
Redundancy | 6.7 | 6.8 |
CC(1/2) | 0.997 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 70% Tacsimate pH 7.0 |