8DAP
[GA/TC] Self-Assembled 3D DNA Tensegrity Triangle with 24 bp Arm Length forming a Trigonal Hexagon
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-12 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 166.609, 166.609, 119.318 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.130 - 6.470 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.17340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.111 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.140 | 6.820 |
| High resolution limit [Å] | 6.470 | 6.470 |
| Number of reflections | 3631 | 203 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 91.1 | |
| Redundancy | 19.7 | |
| CC(1/2) | 0.999 | 0.283 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 1.75 M ammonium sulfate, 120 mM Tris, 120 mM Acetic Acid, 6 mM EDTA |
