8D5N
Crystal structure of Ld-HF10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.503, 139.816, 87.344 |
| Unit cell angles | 90.00, 130.82, 90.00 |
Refinement procedure
| Resolution | 50.005 - 1.800 |
| Rwork | 0.178 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ld9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.738 | |
| Rpim | 0.050 | |
| Number of reflections | 102152 | 17831 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% w/v PEG10K, 0.1M Sodium citrate tribasic dihydrate pH5.5, and 1M Lithium sulfate monohydrate |
