8D59

Crystal structure of human METTL1 in complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 61
Unit cell lengths	86.026, 86.026, 65.579
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.250 - 2.260
R-factor	0.2083
Rwork	0.207
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ckk
RMSD bond length	0.002
RMSD bond angle	0.546
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.250	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.292	1.890
Rpim	0.070	0.797
Number of reflections	12936	579
<I/σ(I)>	13.5	1.2
Completeness [%]	99.2	91.6
Redundancy	15.5	5.7
CC(1/2)	0.983	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	295.15	50 mM Sodium cacodylate pH 5.7, 1.5 M Lithium sulfate, 10 mM Magnesium sulfate