8D58

Crystal structure of human METTL1-WDR4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	79.495, 91.113, 122.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.710 - 2.470
R-factor	0.2045
Rwork	0.203
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vdu
RMSD bond length	0.002
RMSD bond angle	0.436
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.710	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.242	3.760
Rpim	0.072	1.350
Number of reflections	32208	1586
<I/σ(I)>	17.48	0.8
Completeness [%]	99.9	100
Redundancy	11.9	8.2
CC(1/2)	0.998	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	50 mM MES pH 6.0, 6 mM MgCl2, 10 % isopropanol, 30% D-(+)-Galactose, 10 mM L-Glutathione reduced, 10 mM L-Glutathione oxidized