8D3A

Crystal Structure of DH475 Fab in complex with Man9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97920
Spacegroup name	P 61 2 2
Unit cell lengths	126.726, 126.726, 215.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.940 - 2.900
R-factor	0.2312
Rwork	0.229
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpp
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.940	3.080
High resolution limit [Å]	2.900	2.900
Rmerge		1.064
Number of reflections	22928	3672
<I/σ(I)>	8
Completeness [%]	98.0	99.1
Redundancy	3.1
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M (NH4)2SO4, 100 mM PIPES, pH 6.0