8CZQ
The crystal structure of MtbTOP1 in complex with both G- and T-segments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.501, 98.632, 104.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.250 - 2.780 |
| R-factor | 0.2486 |
| Rwork | 0.248 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cqi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.557 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.830 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.092 | 1.372 |
| Rmeas | 0.100 | |
| Rpim | 0.040 | 0.614 |
| Number of reflections | 24480 | 1195 |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 5.8 |
| CC(1/2) | 0.982 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M sodium acetate, trihydrate, 12% (w/v) PEG 3350 |
