8CU0

12-mer DNA structure of ExBIM bound to RNaseH -modified DDD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-07-11
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	36.894, 89.403, 73.890
Unit cell angles	90.00, 101.42, 90.00

Refinement procedure

Resolution	28.490 - 1.740
R-factor	0.2045
Rwork	0.202
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3EY1 (Protein alone)
RMSD bond length	0.008
RMSD bond angle	1.018
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	1.810
High resolution limit [Å]	1.740	1.740
Rmerge	0.106	1.585
Rmeas		1.749
Rpim	0.040	0.716
Number of reflections	47425	4470
<I/σ(I)>	22.11	0.63
Completeness [%]	99.3	93.9
Redundancy	7.8
CC(1/2)		0.327

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate, 30% PEG 8000 (W/V)