8CT8

Crystal structure of Drosophila melanogaster PRL/CBS-pair domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.95374
Spacegroup name	P 41 21 2
Unit cell lengths	83.311, 83.311, 238.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.440 - 2.500
R-factor	0.2285
Rwork	0.225
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k22
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.042	0.284
Rmeas	0.059	0.402
Rpim	0.042	0.284
Number of reflections	54823	5524
<I/σ(I)>	20.04	2.19
Completeness [%]	99.7	99.52
Redundancy	1.8	1.9
CC(1/2)	0.995	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Bis-Tris propane pH 6.5, 17% (wt/vol) PEG3350, 0.2 M NaI