8CR8
human Interleukin-23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.748, 59.842, 69.989 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 69.300 - 2.000 |
| R-factor | 0.2362 |
| Rwork | 0.235 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.525 |
| Data reduction software | XDS (20200131) |
| Data scaling software | XDS (20200131) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.300 | 2.450 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.270 | 2.000 |
| Rmeas | 0.073 | 0.558 | 1.411 |
| Number of reflections | 35521 | 5252 | 4469 |
| <I/σ(I)> | 13.9 | 3.41 | 1.02 |
| Completeness [%] | 94.3 | 99.3 | 74.1 |
| Redundancy | 6.58 | 7.05 | 5.07 |
| CC(1/2) | 0.999 | 0.898 | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | (Morpheus2 E7) 2mM Lanthanides, 0.1M BES/ TEA, 10% PEG8000,20% 1.5-pentanediol |
