8CR6

mouse Interleukin-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.008
Spacegroup name	P 21 21 21
Unit cell lengths	59.110, 70.770, 127.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.340 - 2.850
R-factor	0.2567
Rwork	0.255
R-free	0.29560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.493
Data reduction software	XDS (20170215)
Data scaling software	XSCALE (20170215)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.340	47.340	3.020
High resolution limit [Å]	2.850	8.430	2.850
Rmeas	0.058	0.032	1.090
Number of reflections	12530	521	1975
<I/σ(I)>	13.9	35.8	1.2
Completeness [%]	96.1	91.2	96.4
Redundancy	2.91	2.58	2.88
CC(1/2)	0.999	0.998	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	JCSG-plus G4: 0.2 M TMAO, 0.1 M Tris pH 8.5, 20% w/v/ PEG 2000 MME, 20 mM BaCl2