8CR5

Murine Interleukin-12 receptor beta 1 domain 1 in complex with murine Interleukin-12 beta.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.008
Spacegroup name	P 1 21 1
Unit cell lengths	29.220, 165.090, 51.870
Unit cell angles	90.00, 90.44, 90.00

Refinement procedure

Resolution	49.480 - 2.150
R-factor	0.2432
Rwork	0.241
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.654
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	165.000	2.210
High resolution limit [Å]	2.150	2.150
Rmeas	0.172	1.312
Number of reflections	26490	1969
<I/σ(I)>	7.18	1.02
Completeness [%]	99.5	99.5
Redundancy	3.558	3.687
CC(1/2)	0.992	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	(BCS E4) 20% PEG smear medium, 0.1M MgCl2, 0.1 M KCl, 0.1M PIPES pH 7.0