8COD
Crystal structure of S-adenosyl-L-homocysteine hydrolase from Mus musculus in complex with inosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 98.220, 102.526, 173.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.250 - 2.480 |
| R-factor | 0.14936 |
| Rwork | 0.146 |
| R-free | 0.21625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.568 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.250 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.180 | 0.970 |
| Number of reflections | 31056 | 1520 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 180 mM sodium formate, pH 6.9, and 22% PEG 3350 |
