8CM9
Structure of human O-GlcNAc transferase in complex with UDP and tP11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 272.970, 272.970, 142.593 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 122.100 - 2.800 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.544 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.100 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.286 | 2.647 |
| Rpim | 0.062 | 0.563 |
| Number of reflections | 149701 | 7348 |
| <I/σ(I)> | 9.3 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 21.2 | 22 |
| CC(1/2) | 0.996 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.5 M potassium phosphate dibasic, 5 % xylitol and 10 mM EDTA |
