8CLB

Colchicine bound to tubulin (T2R-TTL) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESA
Synchrotron site	SwissFEL ARAMIS
Beamline	ESA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-02-28
Detector	PSI JUNGFRAU 16M
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	106.710, 160.610, 180.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.180 - 3.000
R-factor	0.1639
Rwork	0.163
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.762
Data reduction software	CrystFEL (0.8.0)
Data scaling software	CrystFEL (0.8.0)
Phasing software	PHENIX (1.20_4487)
Refinement software	PHENIX (1.20_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.810	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	85408	8462
<I/σ(I)>	4.56	0.64
Completeness [%]	100.0
Redundancy	138.4
CC(1/2)	0.944	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	293	6% PEG4000 (w/v), 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine