8CIZ

DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Mycoplanecin A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033202
Spacegroup name	C 1 2 1
Unit cell lengths	83.937, 150.050, 72.381
Unit cell angles	90.00, 103.92, 90.00

Refinement procedure

Resolution	51.280 - 2.270
R-factor	0.2089
Rwork	0.207
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.201-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.030	2.390
High resolution limit [Å]	2.270	2.270
Rmerge	0.115	0.660
Rmeas	0.124	0.716
Rpim	0.046	0.274
Total number of observations	280126	38813
Number of reflections	39802	5767
<I/σ(I)>	6.8	2.3
Completeness [%]	99.0
Redundancy	7	6.7
CC(1/2)	0.995	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.2 M CaCl2 0.15 M MgCl2 8.75 % (v/v) Glycerol 17.5 % (w/v) PEG 3350 0.1 M Tris/HCl pH 9.0 Cryoprotection: 10 % (v/v) (2R,3R)-2,3-butanediol.