8CIR
The FERM domain of human moesin with a bound peptide identified by phage display
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.466, 155.391, 63.607 |
| Unit cell angles | 90.00, 94.28, 90.00 |
Refinement procedure
| Resolution | 77.696 - 1.850 |
| Rwork | 0.178 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.635 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.700 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 74812 | 4621 |
| <I/σ(I)> | 7.8 | 0.9 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 13.2 | |
| CC(1/2) | 0.997 | 0.347 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM sodium bromide, 100 mM bis-tris-propane, 10% ethylene glycol, 20% PEG3350 |
