8CEF
Asymmetric Dimerization in a Transcription Factor Superfamily is Promoted by Allosteric Interactions with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2017-05-22 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.98007 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 167.846, 70.747, 94.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.720 - 2.486 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.840 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.4 (21-NOV-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.720 | 2.810 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 23495 | 1175 |
| <I/σ(I)> | 7.1 | 1.5 |
| Completeness [%] | 93.6 | |
| Redundancy | 13.6 | |
| CC(1/2) | 0.576 | 0.636 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | pH 6.5, PEG, Magnesium chloride |
