8CDS

Crystal structure of the xhNup93-Nb4i VHH antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	26.877, 42.105, 44.946
Unit cell angles	90.00, 93.06, 90.00

Refinement procedure

Resolution	30.710 - 1.530
R-factor	0.1376
Rwork	0.133
R-free	0.17550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.804
Data reduction software	XDS (Jan 31, 2020 BUILT=20200417)
Data scaling software	XSCALE (Jan 31, 2020 BUILT=20200417)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.710	30.710	1.440
High resolution limit [Å]	1.360	4.060	1.360
Rmerge	0.053	0.040	2.489
Rmeas	0.064	0.047	3.071
Number of reflections	40137	1594	6289
<I/σ(I)>	9.94
Completeness [%]	94.5
Redundancy	6.2187
CC(1/2)	0.997	0.997	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.1 M imidazole pH 8, 30% (w/v) PEG 8,000, 0.2 M NaCl