8C7M

Interleukin 12 receptor subunit beta-1 Fn domains in complex with antagonistic FAb fragment.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	73.663, 140.178, 219.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.090 - 2.560
R-factor	0.2041
Rwork	0.203
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS (20210323)
Data scaling software	XDS (20210323)
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.180	140.180	2.710
High resolution limit [Å]	2.560	7.660	2.560
Rmeas	0.104	0.038	2.083
Number of reflections	73952	3002	11706
<I/σ(I)>	19.16	65.94	1.3
Completeness [%]	99.8	99.8	98.9
Redundancy	13.6	12.42	13.285
CC(1/2)	0.999	0.999	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	Morpheus2 condition A3: 90 mM LiNaKSO4, 100 mM Citrate BisTRIS propane pH 5.4, 8.57% PEG 8000, 17.14% 1,5 Pentanediol