8C61
Structure of USP54 in complex with Lys63-linked diUbiquitin-PA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 122.743, 126.559, 144.167 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.280 - 2.500 |
R-factor | 0.2026 |
Rwork | 0.200 |
R-free | 0.24330 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.623 |
Data reduction software | DIALS (3.5.0) |
Data scaling software | Aimless (1.12.10) |
Phasing software | CRANK2 (2.0.281) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.280 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.062 | 1.239 |
Rmeas | 0.066 | 1.313 |
Number of reflections | 149985 | 7759 |
<I/σ(I)> | 16.2 | 1.7 |
Completeness [%] | 100.0 | |
Redundancy | 8.9 | |
CC(1/2) | 0.998 | 0.682 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 27.2 % (v/v) PEG 400, 0.1 M HEPES, 0.2 M calcium chloride |