8C13

Crystal structure of pVHL:ElonginC:ElonginB complex bound to PROTAC JW48

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999987
Spacegroup name	C 1 2 1
Unit cell lengths	73.797, 60.787, 97.228
Unit cell angles	90.00, 100.58, 90.00

Refinement procedure

Resolution	47.833 - 2.300
Rwork	0.223
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.756
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.833	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.111	1.207
Rmeas	0.121	1.299
Rpim	0.046	0.477
Total number of observations		13682
Number of reflections	18781	1874
<I/σ(I)>	9.9	1.8
Completeness [%]	99.1
Redundancy	7	7.3
CC(1/2)	0.997	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	18% PEG 3350, HEPES pH7