8C0J

Structure of AmiB enzymatic domain bound to the EnvC LytM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	I 41 3 2
Unit cell lengths	237.363, 237.363, 237.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.005 - 3.381
Rwork	0.249
R-free	0.29090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in silico model
RMSD bond length	0.008
RMSD bond angle	1.405
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.340	3.650
High resolution limit [Å]	3.380	3.380
Rpim	0.088	0.686
Number of reflections	16294	3305
<I/σ(I)>	9.9	1.6
Completeness [%]	100.0
Redundancy	78.2
CC(1/2)	0.998	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Na Phosphate pH7.6