8BZS

The crystal structure of glycogen phosphorylase in complex with baicalein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-11-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 43 21 2
Unit cell lengths	128.510, 128.510, 116.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.600 - 2.250
R-factor	0.15357
Rwork	0.151
R-free	0.20261
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r0h
RMSD bond length	0.012
RMSD bond angle	1.815
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.600	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.132
Number of reflections	45448	3980
<I/σ(I)>	6.3
Completeness [%]	97.5
Redundancy	3.8
CC(1/2)	0.984	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.8	289	10 mM BES buffer