8BXK

Ntaya virus methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-08
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	P 21 21 21
Unit cell lengths	55.090, 110.060, 116.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.960 - 2.310
R-factor	0.2025
Rwork	0.201
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mrk
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.960	2.390
High resolution limit [Å]	2.310	2.310
Number of reflections	27743	1077
<I/σ(I)>	16.4	3.7
Completeness [%]	87.2
Redundancy	6.3
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	4.0 M Sodium formate