8BWI
Crystal structure of human Twisted gastrulation protein homolog 1 (TWSG1), crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 92.250, 92.250, 95.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.120 - 3.400 |
| R-factor | 0.2977 |
| Rwork | 0.295 |
| R-free | 0.34240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.080 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.251 | 5.961 |
| Rmeas | 0.265 | 6.167 |
| Rpim | 0.069 | 1.551 |
| Number of reflections | 3626 | 254 |
| <I/σ(I)> | 8.2 | 0.6 |
| Completeness [%] | 99.4 | 98.5 |
| Redundancy | 15.6 | 15.4 |
| CC(1/2) | 0.996 | 0.436 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.0 M K/Na tartrate, 0.1 M MES pH 6.0 |
