8BUY

Rac-binding domain of human MiniBAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98008
Spacegroup name	C 1 2 1
Unit cell lengths	44.714, 65.945, 90.438
Unit cell angles	90.00, 91.52, 90.00

Refinement procedure

Resolution	28.330 - 1.600
R-factor	0.1825
Rwork	0.181
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.726
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	Arcimboldo
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.944	1.625
High resolution limit [Å]	1.597	1.597
Number of reflections	34818	1681
<I/σ(I)>	14.8
Completeness [%]	99.9
Redundancy	4.8
CC(1/2)	0.999	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	PEG