8BUX

Rab-binding domain of human MiniBAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 2
Unit cell lengths	60.388, 123.956, 38.068
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.580 - 1.860
R-factor	0.1672
Rwork	0.165
R-free	0.21400
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.052
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	SHELXD
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.580	1.888
High resolution limit [Å]	1.856	1.856
Number of reflections	24856	4668
<I/σ(I)>	10.6
Completeness [%]	99.5
Redundancy	3.6
CC(1/2)	0.996	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	PEG