8BQ8

Crystal structure of Trichoplax Dlg PDZ2 domain in complex with Trichoplax Vangl peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	C 2 2 21
Unit cell lengths	64.673, 101.980, 106.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.340 - 2.700
R-factor	0.225
Rwork	0.222
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g69
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	9739	716
<I/σ(I)>	7.3
Completeness [%]	98.7
Redundancy	5.5
CC(1/2)	0.982	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.2 M ammonium sulphate, 0.1 M Bis-Tris, pH 6.5, 20% glucose