4G69

Structure of the Human Discs Large 1 PDZ2 - Adenomatous Polyposis Coli Cytoskeletal Polarity Complex

Summary for 4G69

• Entry DOI: 10.2210/pdb4g69/pdb
• Descriptor: Disks large homolog 1, Adenomatous polyposis coli protein (3 entities in total)
• Functional Keywords: pdz, maguk, polarity, adenomatous polyposis coli, tetramethylrhodamine, adherens junction, membrane protein-signaling protein complex, membrane protein/signaling protein
• Biological source: Homo sapiens (human); More
• Cellular location: Membrane ; Peripheral membrane protein : Q12959; Cell junction, adherens junction : P25054
• Total number of polymer chains: 2
• Total formula weight: 11807.57

Authors

Slep, K.C. (deposition date: 2012-07-18, release date: 2012-12-12, Last modification date: 2023-09-13)

Primary citation

Slep, K.C.
Structure of the Human Discs Large 1 PDZ2- Adenomatous Polyposis Coli Cytoskeletal Polarity Complex: Insight into Peptide Engagement and PDZ Clustering.
Plos One, 7:e50097-e50097, 2012

PubMed Abstract: The membrane associated guanylate kinase (MAGUK) family member, human Discs Large 1 (hDlg1) uses a PDZ domain array to interact with the polarity determinant, the Adenomatous Polyposis Coli (APC) microtubule plus end binding protein. The hDLG1-APC complex mediates a dynamic attachment between microtubule plus ends and polarized cortical determinants in epithelial cells, stem cells, and neuronal synapses. Using its multi-domain architecture, hDlg1 both scaffolds and regulates the polarity factors it engages. Molecular details underlying the hDlg1-APC interaction and insight into how the hDlg1 PDZ array may cluster and regulate its binding factors remain to be determined. Here, I present the crystal structure of the hDlg1 PDZ2-APC complex and the molecular determinants that mediate APC binding. The hDlg1 PDZ2-APC complex also provides insight into potential modes of ligand-dependent PDZ domain clustering that may parallel Dlg scaffold regulatory mechanisms. The hDlg1 PDZ2-APC complex presented here represents a core biological complex that bridges polarized cortical determinants with the dynamic microtubule cytoskeleton.

PubMed: 23185543
DOI: 10.1371/journal.pone.0050097

Experimental method

X-RAY DIFFRACTION (2 Å)