8BN8
METTL3-METTL14 heterodimer bound to the SAM competitive small molecule inhibitor STM3006
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.970, 63.970, 226.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.493 - 2.213 |
| Rwork | 0.184 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l6d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.945 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.493 | 2.252 |
| High resolution limit [Å] | 2.213 | 2.213 |
| Number of reflections | 27798 | 1337 |
| <I/σ(I)> | 12 | 1.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 | |
| CC(1/2) | 0.999 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 150 MM MAGNESIUM ACETATE, 22% W/V PEG 3350 |
