8BK2

X-ray structure of meningococcal factor H binding protein variant 2 in complex with a specific and bactericidal human monoclonal antibody 1B1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	150
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 1 21 1
Unit cell lengths	77.153, 135.641, 121.530
Unit cell angles	90.00, 105.18, 90.00

Refinement procedure

Resolution	49.660 - 2.410
R-factor	0.2076
Rwork	0.204
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xzw
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.660	2.490
High resolution limit [Å]	2.410	2.410
Number of reflections	92813	9128
<I/σ(I)>	10
Completeness [%]	99.7
Redundancy	3.5
CC(1/2)	0.997	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.09M Sodium Nitrate, Sodium Phosphate dibasic, Ammonium Solphate, 0.1 M Buffer Imidazole, MES monohydrate, pH6.5 and as precipitant mix 25% v/v MPD, 25% PEG 100, 25% w/v PEG 3350.