8BK2

X-ray structure of meningococcal factor H binding protein variant 2 in complex with a specific and bactericidal human monoclonal antibody 1B1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Factor H-binding protein	polymer	260	28217.4	3	UniProt (B9VX96) Pfam (PF20937) Pfam (PF08794) UniProt (by SIFTS) (C6JW56)	Neisseria meningitidis serogroup B
2	D, F, H (H, D, F)	Fab Heavy chain	polymer	226	23871.8	3		Homo sapiens
3	E, G, I (L, E, G)	Fab light Chains	polymer	216	23513.1	3		Homo sapiens
4	J, K, P, Q, R... (A, B, D, E, F)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
5	L (B)	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
6	M (C)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	N (L)	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	1	Chemie (P33)
8	O (L)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
9	T (G)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
10	AA, BA, CA, U, V... (E, F, G, A, B...)	water	water		18.0	213	Chemie (HOH)

Sequence modifications

A, B, C: 12 - 258 (UniProt: B9VX96)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Asp 3	-	expression tag
Ser 4	-	expression tag
Asp 5	-	expression tag
Arg 6	-	expression tag
Leu 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Arg 10	-	expression tag
Arg 11	-	expression tag
Leu 259	-	expression tag
Glu 260	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	226807.0
Non-Polymers*	Number of molecules	11
	Total formula weight	1264.4
All*	Total formula weight	228071.5

*Water molecules are not included.