8BIU
Cystathionine gamma-lyase in complex with cystathionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 157.737, 157.737, 93.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.401 - 1.899 |
| R-factor | 0.1632 |
| Rwork | 0.152 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nmp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.862 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.500 | 1.930 |
| High resolution limit [Å] | 1.899 | 1.900 |
| Number of reflections | 105469 | 5190 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.9 | |
| CC(1/2) | 0.992 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% (w/v) PEG 3350 0.1M sodium citrate pH 4.6, CYMAL-4 |
