8BDG

Tubulin-taxane-2b complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.91501
Spacegroup name	P 21 21 21
Unit cell lengths	105.250, 158.640, 179.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.368 - 2.350
R-factor	0.1845
Rwork	0.183
R-free	0.21390
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.368	49.368	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.176	0.024	4.069
Rmeas	0.178	0.025	4.107
Total number of observations	6869262
Number of reflections	125172	1533	9157
<I/σ(I)>	27.8	165.08	1.24
Completeness [%]	100.0	98.5	100
Redundancy	54.879	50.348	55.235
CC(1/2)	1.000	1.000	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	2-4% PEG 4K, 2-10% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole