8BDF
Tubulin-taxane-2a complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.840, 157.890, 179.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.284 - 1.950 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.21570 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.284 | 49.284 | 2.000 |
High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
Rmerge | 0.133 | 0.027 | 5.166 |
Rmeas | 0.136 | 0.028 | 5.261 |
Total number of observations | 5894682 | ||
Number of reflections | 215803 | 2621 | 15836 |
<I/σ(I)> | 20.05 | 111.21 | 0.73 |
Completeness [%] | 100.0 | 98.7 | 100 |
Redundancy | 27.315 | 25.325 | 27.761 |
CC(1/2) | 1.000 | 1.000 | 0.314 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.7 | 293 | 2-4% PEG 4K, 2-10% glycerol, 30 mM MgCl2, 524 30 mM CaCl2, 0.1 M MES/Imidazole |