8BBY
VarB H/L (SLPL/SLPH) complex from C. difficile SlpA (R20291 strain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-04 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.708459 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.926, 131.291, 138.269 |
Unit cell angles | 90.00, 108.78, 90.00 |
Refinement procedure
Resolution | 36.230 - 2.900 |
R-factor | 0.2459 |
Rwork | 0.244 |
R-free | 0.28690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7acz |
Data reduction software | DIALS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.230 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rpim | 0.086 | 0.404 |
Number of reflections | 40129 | 3584 |
<I/σ(I)> | 8 | 2.19 |
Completeness [%] | 97.8 | 86.49 |
Redundancy | 2 | |
CC(1/2) | 0.978 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.03 M Magnesium chloride hexahydrate; 0.03 M Calcium chloride dihydrate, 0.12 M Ethyleneglycol, 0.05 M Tris (base); 0.05 M BICINE pH 8.5, 20% v/v Glycerol; 10% w/v PEG 4,000 |