8BBQ
Determination of the structure of active tyrosinase from bacterium Verrucomicrobium spinosum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97626 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.813, 63.422, 116.020 |
Unit cell angles | 90.00, 96.83, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 115.200 | 1.560 |
High resolution limit [Å] | 1.430 | 1.430 |
Number of reflections | 62664 | 3134 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 93.4 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v PEG5000MME |