8BBK

Crystal structure of human Sirt3 in complex with a fragment of the human AROS protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 32 2 1
Unit cell lengths	110.215, 110.215, 344.362
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.190 - 3.270
R-factor	0.205
Rwork	0.202
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gls
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	3.380
High resolution limit [Å]	3.270	3.270
Rmerge	0.316
Number of reflections	38569	3207
<I/σ(I)>	7.9
Completeness [%]	99.7
Redundancy	10.9
CC(1/2)	0.997	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	12 % PEG 8.000, 0.1 M HEPES pH 7.5 and 0.2 M NaCl