8B9U

Structure of ClpC1 NTD from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-03-21
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54789
Spacegroup name	P 32 2 1
Unit cell lengths	57.440, 57.440, 174.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.840 - 2.250
R-factor	0.2141
Rwork	0.212
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdb
RMSD bond length	0.002
RMSD bond angle	0.438
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.300
High resolution limit [Å]	2.250	2.250
Rmeas	0.089	0.653
Number of reflections	16532	1084
<I/σ(I)>	19.7
Completeness [%]	98.9	88.2
Redundancy	11.6	10.5
CC(1/2)	0.999	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10 mM Tris-HCl pH 7.5 and 3.1 M sodium formate