8B7D
Luminal domain of TMEM106B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-07 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.408, 52.408, 132.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.760 - 2.590 |
| R-factor | 0.2349 |
| Rwork | 0.234 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alpha fold model Q8N353 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.606 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4682) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.187 | 3.804 |
| Rpim | 0.038 | 0.756 |
| Number of reflections | 6291 | 301 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25 | 25.8 |
| CC(1/2) | 0.371 | 0.371 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2.0 M NaCl, 0.1 M BisTris-HCl, pH 5.5 |
