8B7C
Tubulin-maytansinoid-12 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-23 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000029 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.866, 157.939, 180.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.570 - 1.900 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21150 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.586 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.570 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.119 | 3.954 |
Rmeas | 0.124 | 4.111 |
Rpim | 0.034 | 1.118 |
Number of reflections | 235350 | 23217 |
<I/σ(I)> | 15.92 | 0.58 |
Completeness [%] | 99.9 | 99.81 |
Redundancy | 13.5 | 13.2 |
CC(1/2) | 0.999 | 0.238 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |