8B7B
Tubulin - maytansinoid - 6 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-12 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999995 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.085, 155.637, 181.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.830 - 2.250 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.22430 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.489 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.830 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.149 | 2.913 |
Rmeas | 0.155 | 3.029 |
Rpim | 0.041 | 0.824 |
Number of reflections | 139947 | 13689 |
<I/σ(I)> | 16.76 | 0.86 |
Completeness [%] | 99.4 | 98.84 |
Redundancy | 13.7 | 13.3 |
CC(1/2) | 0.999 | 0.357 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |